Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration
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Date
2016
Authors
Yeadon, Patricia Jane
Bowring, Frederick James
Catcheside, David Edward
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Publisher
Public Library of Science
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Copyrigh: © 2016 Yeadon et al.
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The Authors
Abstract
Analysis of thousands of Δmsh-2 octads using our fluorescent recombination system indicates
that, as in other filamentous fungi, symmetric heteroduplex is common in the his-3
region of Neurospora crassa. Symmetric heteroduplex arises from Holliday junction migration,
and we suggest this mechanism explains the high frequency of His+ spores in heteroallelic
crosses in which recombination is initiated cis to the his-3 allele further from the
initiator, cog+. In contrast, when recombination is initiated cis to the his-3 allele closer to
cog+, His+ spores are mainly a result of synthesis-dependent strand annealing, yielding
asymmetric heteroduplex. Loss of Msh-2 function increases measures of allelic recombination
in both his-3 and the fluorescent marker gene, indicating that mismatches in asymmetric
heteroduplex, as in Saccharomyces cerevisiae, tend to be repaired in the direction of
restoration. Furthermore, the presence of substantial numbers of conversion octads in
crosses lacking Msh-2 function suggests that the disjunction pathway described in S. cerevisiae
is also active in Neurospora, adding to evidence for a universal model for meiotic
recombination.
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Yeadon PJ, Bowring FJ, Catcheside DEA (2016) Meiotic Recombination in Neurospora crassa Proceeds by Two Pathways with Extensive Holliday Junction Migration. PLoS ONE 11(1): e0147815. doi:10.1371/journal.pone.0147815