A simple corneal perfusion chamber for drug penetration and toxicity studies

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dc.contributor.authorThiel, Michael A
dc.contributor.authorMorlet, N
dc.contributor.authorSchulz, D
dc.contributor.authorEdelhauser, HF
dc.contributor.authorDart, JK
dc.contributor.authorCoster, Douglas John
dc.contributor.authorWilliams, Keryn Anne
dc.date.accessioned2006-06-20T03:23:58Z
dc.date.available2006-06-20T03:23:58Z
dc.date.issued2001-04
dc.description.abstractAIMS: Corneal perfusion chambers are important tools in the development and assessment of ophthalmic drugs. The aim of this study was to design and test a modified perfusion chamber suitable for topical application of drugs to isolated corneoscleral preparations, and which allowed continuous monitoring of endothelial cell function. METHODS: A polycarbonate and stainless steel perfusion chamber was designed to clamp corneas in a horizontal plane suitable for topical drug delivery. Endothelial cell function was assessed by ultrasonic pachymetry and specular microscopy during perfusion. Epithelial barrier function was assessed by penetration of fluorescein. Leakage was examined by measuring penetration of a large protein, IgG. Tissue architecture after perfusion was examined by conventional histology. RESULTS: Corneas maintained a functionally and morphologically intact endothelial monolayer during perfusion periods of up to 14 hours. The epithelial barrier function was well preserved. The tissue clamp sealed the preparation effectively against leakage of macromolecules. CONCLUSION: The new chamber device forms a reliable tool for in vitro drug penetration and toxicity studies in isolated perfused corneoscleral tissue.en
dc.format.extent169365 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationThiel, M.A Morlet, N. Schulz, D. Edelhauser, H.F. Dart, J.K. Coster, D.J Williams, K.A. 2001 A simple corneal perfusion chamber for drug penetration an toxicity studies. 'British Journal of Ophthalmology' Vol. 85, No. 4, 450-453en
dc.identifier.issn0007-1161
dc.identifier.urihttp://hdl.handle.net/2328/1001
dc.language.isoen
dc.oaire.license.condition.licenseIn Copyright
dc.publisherBMJ Publishing Group - http://bjo.bmjjournals.com/en
dc.subjectDiffusion Chambers, Cultureen
dc.subjectDrug Evaluation, Preclinicalen
dc.subjectEndothelium, Cornealen
dc.subjectEquipment Designen
dc.subjectFluoresceinen
dc.subjectImmunoglobulin Gen
dc.subjectOphthalmic Solutionsen
dc.subjectPerfusionen
dc.subjectResearch Support, Non-U.S. Gov'ten
dc.subject.otherAustralian Standard Research Classification 321016 Ophthalmology and Vision Scienceen
dc.titleA simple corneal perfusion chamber for drug penetration and toxicity studiesen
dc.typeArticleen
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