Apoptosis is a prominent feature of acute anterior uveitis in the Fischer 344 rat

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Date
2000-02
Authors
Smith, Justine R
Hart, Prue
Standfield, Scott D
Coster, Douglas John
Wing, Sarah J
Williams, Keryn Anne
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BMJ Publishing Group - http://bjo.bmjjournals.com/
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Abstract
AIMS: To examine the hypothesis that apoptosis of infiltrating cells contributes to spontaneous resolution of uveitis in clinically relevant rodent models. METHODS: Experimental melanin induced uveitis (EMIU) was induced in Fischer 344 rats by immunisation with 250 microg bovine ocular melanin. Endotoxin induced uveitis (EIU) was induced by injection of 200 microg Escherichia coli lipopolysaccharide. Formalin fixed, paraffin embedded ocular cross sections were stained by terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate biotin nick end labelling (TUNEL) to identify apoptotic cells. Indirect immunoperoxidase staining of paraformaldehyde lysine periodate fixed tissue cross sections was used to demonstrate expression of inducible nitric oxide synthase (iNOS). RESULTS: TUNEL positive mononuclear cells were observed in the anterior uvea during both EMIU and EIU at all selected time points. However, whereas the majority of mononuclear cells appeared apoptotic from the outset of disease, neutrophils were notably TUNEL negative at all time points examined. Many infiltrating neutrophils expressed iNOS. CONCLUSION: Apoptosis occurs early in the course of rat EMIU and EIU, and may contribute to resolution of these diseases. In general, infiltrating mononuclear cells die rapidly, while neutrophils survive, producing inducible nitric oxide synthase which may contribute to disease pathogenesis.
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Keywords
Apoptosis, Eye Diseases, In Situ Nick-End Labeling, Neutrophils, Nitric Oxide Synthase, Rats, Inbred F344, Uveitis, Anterior
Citation
J R Smith, P H Hart, S D Standfield, D J Coster, S J Wing and K A Williams 2000. Apoptosis is a prominent feature of acute anterior uveitis in the Fischer 344 rat. 'British Journal of Ophthalmology', Feb, Vol 84, No 2, 205-11