Ultrastructural organization of elastic fibres in the partition boundaries of the annulus fibrosus within the intervertebral disc

dc.contributor.author Tavakoli, Javad
dc.contributor.author Costi, John Jack
dc.date.accessioned 2018-04-09T03:02:49Z
dc.date.available 2018-04-09T03:02:49Z
dc.date.issued 2017-12-19
dc.description © 2017 Acta Materialia Inc. Published by Elsevier Ltd. This manuscript version is made available under the CC-BY-NCND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/ This author accepted manuscript is made available following 24 month embargo from date of publication (Dec 2017) in accordance with the publisher’s archiving policy en_US
dc.description.abstract The relationship between elastic fibre disorders and disc degeneration, aging and progression of spine deformity have been discussed in a small number of studies. However, the clinical relevance of elastic fibres in the annulus fibrosus (AF) of the disc is poorly understood. Ultrastructural visualization of elastic fibres is an important step towards understanding their structure-function relationship. In our previous studies, a novel technique for visualization of elastic fibres across the AF was presented and their ultrastructural organization in intra- and inter-lamellar regions was compared. Using the same novel technique in the present study, the ultrastructural organization of elastic fibres in the partition boundaries (PBs), which are located between adjacent collagen bundles, is presented for the first time. Visualization of elastic fibres in the PBs in control and partially digested (digested) samples was compared, and their orientation in two different cutting planes (transverse and oblique) were discussed. The ultrastructural analysis revealed that elastic fibres in PBs were a well-organized dense and complex network having different size and shape. Adjacent collagen bundles in a cross section (CS) lamella appear to be connected to each other, where elastic fibres in the PBs were merged in parallel or penetrated into the collagen bundles. There was no significant difference in directional coherency coefficient of elastic fibres between the two different cutting planes (p = .35). The present study revealed that a continuous network of elastic fibres may provide disc integrity by connecting adjacent bundles of CS lamellae together. Compared to our previous studies, the density of the elastic fibre network in PBs was lower, and fibre orientation was similar to the intra-lamellar space and inter-lamellar matrix. Statement of Significance A detailed ultrastructural study in the partition boundaries of the annulus fibrosus within the disc revealed a well-organized elastic fibre network with a complex ultrastructure. The continuous network of elastic fibres may provide disc integrity by connecting adjacent bundles of cross section lamellae together. The density of the elastic fibre network in PBs was lower, and fibre orientation was similar to the intra-lamellar space and the inter-lamellar matrix. en_US
dc.identifier.citation Tavakoli, J., & Costi, J. J. (2018). Ultrastructural organization of elastic fibres in the partition boundaries of the annulus fibrosus within the intervertebral disc. Acta Biomaterialia, 68, 67–77. https://doi.org/10.1016/ j.actbio.2017.12.017 en_US
dc.identifier.doi https://doi.org/10.1016/j.actbio.2017.12.017 en
dc.identifier.issn 1742-7061
dc.identifier.uri http://hdl.handle.net/2328/37889
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. en_US
dc.rights.holder Acta Materialia Inc. en_US
dc.rights.license CC-BY-NC-ND
dc.subject Partition boundaries en_US
dc.subject Elastic fibres en_US
dc.subject Ultrastructural organization en_US
dc.subject Annulus fibrosus en_US
dc.subject Fibre orientation en_US
dc.title Ultrastructural organization of elastic fibres in the partition boundaries of the annulus fibrosus within the intervertebral disc en_US
dc.type Article en
local.contributor.authorOrcidLookup Tavakoli, Javad: https://orcid.org/0000-0002-0696-6530 en_US
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