Secreted human Ro52 autoantibody proteomes express a restricted set of public clonotypes

dc.contributor.author Arentz, Georgia
dc.contributor.author Thurgood, Lauren Alexandra
dc.contributor.author Lindop, Rhianna
dc.contributor.author Chataway, Timothy Kennion
dc.contributor.author Gordon, Thomas Paul
dc.date.accessioned 2013-08-26T04:52:36Z
dc.date.available 2013-08-26T04:52:36Z
dc.date.issued Dec-12
dc.date.issued 2012-12-01
dc.description This article appeared in a journal published by Elsevier Ltd. Under Elsevier's copyright, mandated authors are not permitted to make work available in an institutional repository. en
dc.description.abstract Long-lived secreted autoantibody responses in systemic autoimmunity are generally regarded to be polyclonal and to express a diverse B-cell repertoire. Here, we have used a proteomic approach based on de novo sequencing to determine the clonality and V region structures of human autoantibodies directed against a prototypic systemic autoantigen, Ro52 (TRIM21). Remarkably, anti-Ro52 autoantibodies from patients with primary Sjögren’s syndrome, systemic lupus erythematosus, systemic sclerosis or polymyositis were restricted to two IgG1 kappa clonotypes that migrated as a single species on isoelectric focusing; shared a common light chain paired with one of two closely-related heavy chains; and were public in unrelated patients. Targeted mass spectrometry using these uniquely mutated V region peptides as surrogates detected anti-Ro52 autoantibodies in human sera with high sensitivity and specificity compared with traditional ELISA. Mass spectrometry-based detection of specific autoantibody motifs provides a powerful new tool for analysis of humoral autoimmunity. en
dc.description.sponsorship Australian National Health & Medical Research Council en
dc.identifier.citation Arentz, G., Thurgood, L.A., Lindop, R., Chataway, T.K. and Gordon, T.P., 2012. Secreted human Ro52 autoantibody proteomes express a restricted set of public clonotypes. Journal of Autoimmunity, 39(4), 466-470. en
dc.identifier.doi https://doi.org/10.1016/j.jaut.2012.07.003 en
dc.identifier.issn 0896-8411
dc.identifier.uri http://hdl.handle.net/2328/27000
dc.language.iso en en_US
dc.publisher Elsevier en
dc.relation http://purl.org/au-research/grants/nhmrc/595907
dc.relation.grantnumber NHMRC/595907
dc.rights Copyright 2012 Elsevier Ltd. Published by Elsevier Ltd. All rights reserved.
dc.rights.holder Elsevier Ltd.
dc.subject Autoimmunity
dc.subject Proteome
dc.subject Autoantibodies
dc.title Secreted human Ro52 autoantibody proteomes express a restricted set of public clonotypes en_US
dc.type Article en
local.contributor.authorOrcidLookup Thurgood, Lauren Alexandra: https://orcid.org/0000-0002-6183-2797 en_US
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