Secreted human Ro52 autoantibody proteomes express a restricted set of public clonotypes

dc.contributor.authorArentz, Georgia
dc.contributor.authorThurgood, Lauren Alexandra
dc.contributor.authorLindop, Rhianna
dc.contributor.authorChataway, Timothy Kennion
dc.contributor.authorGordon, Thomas Paul
dc.descriptionThis article appeared in a journal published by Elsevier Ltd. Under Elsevier's copyright, mandated authors are not permitted to make work available in an institutional repository.en
dc.description.abstractLong-lived secreted autoantibody responses in systemic autoimmunity are generally regarded to be polyclonal and to express a diverse B-cell repertoire. Here, we have used a proteomic approach based on de novo sequencing to determine the clonality and V region structures of human autoantibodies directed against a prototypic systemic autoantigen, Ro52 (TRIM21). Remarkably, anti-Ro52 autoantibodies from patients with primary Sjögren’s syndrome, systemic lupus erythematosus, systemic sclerosis or polymyositis were restricted to two IgG1 kappa clonotypes that migrated as a single species on isoelectric focusing; shared a common light chain paired with one of two closely-related heavy chains; and were public in unrelated patients. Targeted mass spectrometry using these uniquely mutated V region peptides as surrogates detected anti-Ro52 autoantibodies in human sera with high sensitivity and specificity compared with traditional ELISA. Mass spectrometry-based detection of specific autoantibody motifs provides a powerful new tool for analysis of humoral autoimmunity.en
dc.description.sponsorshipAustralian National Health & Medical Research Councilen
dc.identifier.citationArentz, G., Thurgood, L.A., Lindop, R., Chataway, T.K. and Gordon, T.P., 2012. Secreted human Ro52 autoantibody proteomes express a restricted set of public clonotypes. Journal of Autoimmunity, 39(4), 466-470.en
dc.rightsCopyright 2012 Elsevier Ltd. Published by Elsevier Ltd. All rights reserved.
dc.rights.holderElsevier Ltd.
dc.titleSecreted human Ro52 autoantibody proteomes express a restricted set of public clonotypesen_US
local.contributor.authorOrcidLookupThurgood, Lauren Alexandra: