The Microtubule-Modulating Drug Epothilone D Alters Dendritic Spine Morphology in a Mouse Model of Mild Traumatic Brain Injury

dc.contributor.author Chuckowree, Jyoti A
dc.contributor.author Zhu, Zhendan
dc.contributor.author Brizuela, Mariana
dc.contributor.author Lee, Ka M
dc.contributor.author Blizzard, Catherine A
dc.contributor.author Dickson, Tracey C
dc.date.accessioned 2018-09-14T00:02:52Z
dc.date.available 2018-09-14T00:02:52Z
dc.date.issued 2018-07-30
dc.description This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en_US
dc.description.abstract Microtubule dynamics underpin a plethora of roles involved in the intricate development, structure, function, and maintenance of the central nervous system. Within the injured brain, microtubules are vulnerable to misalignment and dissolution in neurons and have been implicated in injury-induced glial responses and adaptive neuroplasticity in the aftermath of injury. Unfortunately, there is a current lack of therapeutic options for treating traumatic brain injury (TBI). Thus, using a clinically relevant model of mild TBI, lateral fluid percussion injury (FPI) in adult male Thy1-YFPH mice, we investigated the potential therapeutic effects of the brain-penetrant microtubule-stabilizing agent, epothilone D. At 7 days following a single mild lateral FPI the ipsilateral hemisphere was characterized by mild astroglial activation and a stereotypical and widespread pattern of axonal damage in the internal and external capsule white matter tracts. These alterations occurred in the absence of other overt signs of trauma: there were no alterations in cortical thickness or in the number of cortical projection neurons, axons or dendrites expressing YFP. Interestingly, a single low dose of epothilone D administered immediately following FPI (and sham-operation) caused significant alterations in the dendritic spines of layer 5 cortical projection neurons, while the astroglial response and axonal pathology were unaffected. Specifically, spine length was significantly decreased, whereas the density of mushroom spines was significantly increased following epothilone D treatment. Together, these findings have implications for the use of microtubule stabilizing agents in manipulating injury-induced synaptic plasticity and indicate that further study into the viability of microtubule stabilization as a therapeutic strategy in combating TBI is warranted. en_US
dc.description.sponsorship This research was supported by the National Health and Medical Research Council of Australia, Select Foundation, Brain Foundation Australia, Motor Accident Insurance Board Tasmania, Flack Foundation, Internal Research Grant Scheme (University of Tasmania), and Tasmanian Masonic Centenary Medical Research Foundation. en_US
dc.identifier.citation Chuckowree, J.A., Zhu, Z., Brizuela, M., Lee, K.M., Blizzard, C.A. and Dickson, T.C., (2018). The Microtubule-Modulating Drug Epothilone D Alters Dendritic Spine Morphology in a Mouse Model of Mild Traumatic Brain Injury. Front. Cell. Neurosci. 12:223. doi: 10.3389/fncel.2018.00223 en_US
dc.identifier.doi https://doi.org/10.3389/fncel.2018.00223 en_US
dc.identifier.issn 1662-5102
dc.identifier.uri http://hdl.handle.net/2328/38277
dc.language.iso en en_US
dc.publisher Frontiers Media en_US
dc.rights Copyright © 2018 Chuckowree, Zhu, Brizuela, Lee, Blizzard and Dickson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en_US
dc.rights.holder Copyright © 2018 Chuckowree, Zhu, Brizuela, Lee, Blizzard and Dickson. en_US
dc.rights.license CC-BY
dc.subject traumatic brain injury en_US
dc.subject fluid percussion injury en_US
dc.subject neuroplasticity en_US
dc.subject microtubule stabilization en_US
dc.subject epothilone D en_US
dc.subject dendritic spine en_US
dc.subject cortical projection neuron en_US
dc.subject mushroom spine en_US
dc.title The Microtubule-Modulating Drug Epothilone D Alters Dendritic Spine Morphology in a Mouse Model of Mild Traumatic Brain Injury en_US
dc.type Article en
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