MALDI MS imaging analysis of apolipoprotein E and lysyl oxidase-like 1 in human lens capsules affected by pseudoexfoliation syndrome
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Date
2013-04-01
Authors
Ronci, M
Sharma, Shiwani
Martin, Sarah
Craig, Jamie E
Voelcker, Nicolas Hans
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Rights
Copyright 2013�Elsevier B.V.�All rights reserved.
Rights Holder
Elsevier, Ltd.
Abstract
Pseudoexfoliation (PEX) syndrome is an age-related systemic disease of the extracellular matrix, characterized by the presence of amyloid-like fibrillar deposits on the anterior lens capsule. The pathological deposits (PEX material) can obstruct aqueous outflow leading to increased intraocular pressure that in turn can result in glaucoma. PEX syndrome is the most common risk factor for glaucoma. In our previous work, we reported a protocol for the analysis of human lens capsules by MALDI MS imaging. Here, we extend our previous work applying the developed protocol to the analysis of human lens capsules affected by PEX syndrome. We focus our investigation on known components of the PEX material, namely lysyl oxidase-like 1 (LOXL1) and apolipoprotein E (APOE). Our results show that LOXL1 is more abundant in the deposits in the iris region and, alternatively APOE is concentrated in the PEX material accumulated in the pupillary area of the anterior lens capsule. Furthermore, we identify potentially relevant post-translational modifications which may have an important role in promoting the cross-linking processes in PEX syndrome and stabilize aggregate structures within the proteinaceous PEX material.
Description
This article appeared in a journal published by Elsevier Ltd. Under Elsevier's copyright, mandated authors are not permitted to make work available in an institutional repository.
Keywords
Pseudoexfoliation syndrome, Glaucoma, Optometry
Citation
Ronci, M., Sharma, S., Martin, S., Craig, J.E. and Voelcker, N.H., 2013. MALDI MS imaging analysis of apolipoprotein E and lysyl oxidase-like 1 in human lens capsules affected by pseudoexfoliation syndrome. Journal of Proteomics, 82, 27-34.