Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense

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dc.contributor.author Kong, Youqin
dc.contributor.author Chen, Li Qiao
dc.contributor.author Ding, Zhili
dc.contributor.author Qin, Jianguang
dc.contributor.author Sun, Shengming
dc.contributor.author Wang, Ligai
dc.contributor.author Ye, Jinyun
dc.date.accessioned 2016-12-05T02:52:50Z
dc.date.available 2016-12-05T02:52:50Z
dc.date.issued 2016-10-13
dc.description This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. en
dc.description.abstract Hemocyanin is a copper-containing protein with immune function against disease. In this study, a hemocyanin subunit named MnHc-1 was cloned from Macrobrachium nipponense. The full-length cDNA of MnHc-1 was 2,163 bp with a 2,028-bp open reading frame (ORF) encoding a polypeptide of 675 amino acids. The MnHc-1 mRNA was expressed in the hepatopancreas, gill, hemocytes, intestine, ovary, and stomach, with the highest level in the hepatopancreas. In the infection trial, the MnHc-1 mRNA transcripts in the hemocytes were significantly downregulated at 3 h after injection of Aeromonas hydrophila and then upregulated at 6 h and 12 h, followed by a gradual recovery from 24 to 48 h. The MnHc-1 transcriptional expression in the hepatopancreas was measured after M. nipponense were fed seven diets with 2.8, 12.2, 20.9, 29.8, 43.1, 78.9, and 157.1 mg Cu kg−1 for 8 weeks, respectively. The level of MnHc-1 mRNA was significantly higher in the prawns fed 43.1–157.1 mg Cu kg−1 diet than in that fed 2.8–29.8 mg Cu kg−1 diet. This study indicated that the MnHc-1 expression can be affected by dietary copper and the hemocyanin may potentially participate in the antibacterial defense of M. nipponense. en
dc.identifier.citation Kong, Y., Chen, L., Ding, Z., Qin, J., Sun, S., Wang, L., & Ye, J. (2016). Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense. International Journal of Genomics, 2016, 6404817. en
dc.identifier.doi https://doi.org/10.1155/2016/6404817 en
dc.identifier.issn 2314-436X
dc.identifier.uri http://hdl.handle.net/2328/36816
dc.language.iso en
dc.oaire.license.condition.license CC-BY
dc.publisher Hindawi Publishing Corporation en
dc.rights Copyright © 2016 The authors en
dc.rights.holder The authors en
dc.subject Molecular Cloning en
dc.subject mRNA en
dc.subject Macrobrachium nipponense en
dc.title Molecular Cloning, Characterization, and mRNA Expression of Hemocyanin Subunit in Oriental River Prawn Macrobrachium nipponense en
dc.type Article en
local.contributor.authorOrcidLookup Qin, Jianguang: http://orcid.org/0000-0003-2448-8058 en_US
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