Selective co-expression of synaptic proteins, α-synuclein, cysteine string protein-α, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in the guinea-pig ileum

dc.contributor.author Sharrad, DF
dc.contributor.author Gai, Wei Ping
dc.contributor.author Brookes, Simon Jonathan
dc.date.accessioned 2013-08-26T04:52:34Z
dc.date.available 2013-08-26T04:52:34Z
dc.date.issued 2013-06-04
dc.description Author version made available in accordance with publisher's policy. en
dc.description.abstract Parkinson's disease is a neurodegenerative disorder characterized by Lewy bodies and neurites composed mainly of the presynaptic protein α-synuclein. Frequently, Lewy bodies and neurites are identified in the gut of Parkinson's disease patients and may underlie associated gastrointestinal dysfunctions. We recently reported selective expression of α-synuclein in the axons of cholinergic neurons in the guinea pig and human distal gut; however, it is not clear whether α-synuclein expression varies along the gut, nor how closely expression is associated with other synaptic proteins. We used multiple-labeling immunohistochemistry to quantify which neurons in the guinea pig ileum expressed α-synuclein, cysteine string protein-α (CSPα), synaptophysin, synaptotagmin-1, or synaptobrevin-2 in their axons. Among the 10 neurochemically defined axonal populations, a significantly greater proportion of vesicular acetylcholine transporter-immunoreactive (VAChT-IR) varicosities (80% ± 1.7%, n = 4, P < 0.001) contained α-synuclein immunoreactivity, and a significantly greater proportion of α-synuclein-IR axons also contained VAChT immunoreactivity (78% ± 1.3%, n = 4) compared with any of the other nine populations (P < 0.001). Among synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPα-IR varicosities, 98% ± 0.7%, 96% ± 0.7%, 88% ± 1.6%, and 85% ± 2.9% (n = 4) contained α-synuclein immunoreactivity, respectively. Among α-synuclein-IR varicosities, 96% ± 0.9%, 99% ± 0.6%, 83% ± 1.9%, and 87% ± 2.3% (n = 4) contained synaptophysin-, synaptotagmin-1-, synaptobrevin-2-, and CSPα immunoreactivity, respectively. We report a close association between the expression of α-synuclein and the expression of other synaptic proteins in cholinergic axons in the guinea pig ileum. Selective expression of α-synuclein may relate to the neurotransmitter system utilized and predispose cholinergic enteric neurons to degeneration in Parkinson's disease. en
dc.description.sponsorship Australian National Health & Medical Research Council en
dc.identifier.citation Sharrad D.F., Gai, W.P., Brookes, S.J.H., 2013. Selective co-expression of synaptic proteins, α-synuclein, cysteine string protein-α, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in the guinea-pig ileum. Journal of Comparative Neurology, 521(11), 2523-2537. en
dc.identifier.doi https://doi.org/10.1002/cne.23296 en
dc.identifier.issn 0021-9967
dc.identifier.uri http://hdl.handle.net/2328/26991
dc.language.iso en
dc.oaire.license.condition.license In Copyright
dc.publisher Wiley-Blackwell en
dc.relation http://purl.org/au-research/grants/nhmrc/595969 en
dc.relation.grantnumber NHMRC/595969 en
dc.rights Copyright 2013 Wiley Periodicals, Inc. en
dc.rights.holder Wiley-Blackwell en
dc.subject Parkinson's disease en
dc.subject Predisposition en
dc.subject Research en
dc.title Selective co-expression of synaptic proteins, α-synuclein, cysteine string protein-α, synaptophysin, synaptotagmin-1, and synaptobrevin-2 in vesicular acetylcholine transporter-immunoreactive axons in the guinea-pig ileum en
dc.type Article en
local.contributor.authorOrcidLookup Brookes, Simon Jonathan: https://orcid.org/0000-0001-5635-0876 en_US
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