Quantitation of DNA methylation by melt curve analysis
Quantitation of DNA methylation by melt curve analysis
| dc.contributor.author | Smith, Eric | |
| dc.contributor.author | Jones, Michael Emlyn | |
| dc.contributor.author | Drew, Paul Anthony | |
| dc.date.accessioned | 2012-10-02T03:14:56Z | |
| dc.date.available | 2012-10-02T03:14:56Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | We have validated a rapid, simple in-tube method to quantify methylation which is robust and reproducible, utilizes easily designed primers and does not need proprietary algorithms or software. The technique does not depend on any operator manipulation or interpretation of the melt curves, and is suitable for use in any laboratory with a real-time thermocycler. The parameters derived provide an objective description and quantitation of the methylation in a specimen, and can be used for statistical comparisons of methylation between specimens. | en |
| dc.identifier.citation | Smith, E., Jones, M.E., & Drew, P.A., 2009. Quantitation of DNA methylation by melt curve analysis. BMC Cancer, 9:123 (24 April 2009). | en |
| dc.identifier.doi | https://doi.org/10.1186/1471-2407-9-123 | en |
| dc.identifier.issn | 1471-2407 | |
| dc.identifier.rmid | 2006013533 | |
| dc.identifier.uri | http://hdl.handle.net/2328/26328 | |
| dc.language.iso | en | |
| dc.publisher | BioMed Central Ltd. | en |
| dc.rights.license | CC-BY | |
| dc.subject | Medicine | en |
| dc.subject | Public health | en |
| dc.subject | Cancer | en |
| dc.subject | DNA methylation | en |
| dc.title | Quantitation of DNA methylation by melt curve analysis | en |
| dc.type | Article | en |
| local.contributor.authorOrcidLookup | Drew, Paul Anthony: https://orcid.org/0000-0001-5661-4771 | en_US |