Quantitation of DNA methylation by melt curve analysis

dc.contributor.author Smith, Eric
dc.contributor.author Jones, Michael Emlyn
dc.contributor.author Drew, Paul Anthony
dc.date.accessioned 2012-10-02T03:14:56Z
dc.date.available 2012-10-02T03:14:56Z
dc.date.issued 2009
dc.description.abstract We have validated a rapid, simple in-tube method to quantify methylation which is robust and reproducible, utilizes easily designed primers and does not need proprietary algorithms or software. The technique does not depend on any operator manipulation or interpretation of the melt curves, and is suitable for use in any laboratory with a real-time thermocycler. The parameters derived provide an objective description and quantitation of the methylation in a specimen, and can be used for statistical comparisons of methylation between specimens. en
dc.identifier.citation Smith, E., Jones, M.E., & Drew, P.A., 2009. Quantitation of DNA methylation by melt curve analysis. BMC Cancer, 9:123 (24 April 2009). en
dc.identifier.doi https://doi.org/10.1186/1471-2407-9-123 en
dc.identifier.issn 1471-2407
dc.identifier.rmid 2006013533
dc.identifier.uri http://hdl.handle.net/2328/26328
dc.language.iso en
dc.publisher BioMed Central Ltd. en
dc.rights.license CC-BY
dc.subject Medicine en
dc.subject Public health en
dc.subject Cancer en
dc.subject DNA methylation en
dc.title Quantitation of DNA methylation by melt curve analysis en
dc.type Article en
local.contributor.authorOrcidLookup Drew, Paul Anthony: https://orcid.org/0000-0001-5661-4771 en_US
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