Quantitation of DNA methylation by melt curve analysis
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Date
2009
Authors
Smith, Eric
Jones, Michael Emlyn
Drew, Paul Anthony
Journal Title
Journal ISSN
Volume Title
Publisher
BioMed Central Ltd.
Rights
Rights Holder
Abstract
We have validated a rapid, simple in-tube method to quantify methylation which is
robust and reproducible, utilizes easily designed primers and does not need proprietary algorithms
or software. The technique does not depend on any operator manipulation or interpretation of the
melt curves, and is suitable for use in any laboratory with a real-time thermocycler. The parameters
derived provide an objective description and quantitation of the methylation in a specimen, and can
be used for statistical comparisons of methylation between specimens.
Description
Keywords
Medicine, Public health, Cancer, DNA methylation
Citation
Smith, E., Jones, M.E., & Drew, P.A., 2009. Quantitation of DNA methylation by melt curve analysis. BMC Cancer, 9:123 (24 April 2009).