Promotility Action of the Probiotic Bifidobacterium lactis HN019 Extract Compared with Prucalopride in Isolated Rat Large Intestine

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dc.contributor.author Dalziel, Julie E
dc.contributor.author Anderson, Rachel C
dc.contributor.author Peters, Jason S
dc.contributor.author Lynch, Amy T
dc.contributor.author Spencer, Nicholas John
dc.contributor.author Dekker, James
dc.contributor.author Roy, Nicole C
dc.date.accessioned 2017-04-06T03:41:54Z
dc.date.available 2017-04-06T03:41:54Z
dc.date.issued 2017-01-26
dc.description Copyright © 2017 Dalziel, Anderson, Peters, Lynch, Spencer, Dekker and Roy. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.description.abstract Attention is increasingly being focussed on probiotics as potential agents to restore or improve gastrointestinal (GI) transit. Determining mechanism of action would support robust health claims. The probiotic bacterium Bifidobacterium lactis HN019 reduces transit time, but its mechanisms of action and effects on motility patterns are poorly understood. The aim of this study was to investigate changes in GI motility induced by an extract of HN019 on distinct patterns of colonic motility in isolated rat large intestine, compared with a known promotility modulator, prucalopride. The large intestines from male Sprague Dawley rats (3–6 months) were perfused with Kreb's buffer at 37°C in an oxygenated tissue bath. Isometric force transducers recorded changes in circular muscle activity at four independent locations assessing contractile propagation between the proximal colon and the rectum. HN019 extract was perfused through the tissue bath and differences in tension and frequency quantified relative to pre-treatment controls. Prucalopride (1 μM) increased the frequency of propagating contractions (by 75 ± 26%) in the majority of preparations studied (10/12), concurrently decreasing the frequency of non-propagating contractions (by 50 ± 11%). HN019 extract had no effect on contractile activity during exposure (n = 8). However, following wash out, contraction amplitude of propagating contractions increased (by 55 ± 18%) in the distal colon, while the frequency of non-propagating proximal contractions decreased by 57 ± 7%. The prokinetic action of prucalopride increased the frequency of synchronous contractions along the length of colon, likely explaining increased colonic rate of transit in vivo. HN019 extract modified motility patterns in a different manner by promoting propagating contractile amplitude and inhibiting non-propagations, also demonstrating prokinetic activity consistent with the reduction of constipation by B. lactis HN019 in humans. en
dc.identifier.citation Dalziel JE, Anderson RC, Peters JS, Lynch AT, Spencer NJ, Dekker J and Roy NC (2017) Promotility Action of the Probiotic Bifidobacterium lactis HN019 Extract Compared with Prucalopride in Isolated Rat Large Intestine. Front. Neurosci. 11:20. doi: 10.3389/fnins.2017.00020 en
dc.identifier.doi https://doi.org/10.3389/fnins.2017.00020 en
dc.identifier.issn 1662-5161
dc.identifier.uri http://hdl.handle.net/2328/37105
dc.language.iso en
dc.oaire.license.condition.license CC-BY
dc.publisher Frontiers Media S.A. en
dc.rights Copyright © 2017 Dalziel, Anderson, Peters, Lynch, Spencer, Dekker and Roy. en
dc.rights.holder Dalziel, Anderson, Peters, Lynch, Spencer, Dekker and Roy. en
dc.subject serotonin agonist en
dc.subject constipation en
dc.subject colon en
dc.subject motility en
dc.subject contraction en
dc.subject enteric nervoussystem en
dc.title Promotility Action of the Probiotic Bifidobacterium lactis HN019 Extract Compared with Prucalopride in Isolated Rat Large Intestine en
dc.type Article en
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