Visualising latent DNA on swabs

dc.contributor.author Kanokwongnuwut, Piyamas
dc.contributor.author Kirkbride, Kenneth Paul
dc.contributor.author Linacre, Adrian Matthew Thornton
dc.date.accessioned 2018-10-11T04:31:29Z
dc.date.available 2018-10-11T04:31:29Z
dc.date.issued 2018-08-25
dc.description This manuscript version is made available under the CC-BY-NC-ND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/ which permits unrestricted re-use, distribution, and reproduction in any medium, provided the original work is properly cited. This author accepted manuscript is made available following 12 month embargo from date of publication (August 2018) in accordance with the publisher’s archiving policy en_US
dc.description.abstract Collection for touch DNA either at scenes or on items submitted to a forensic laboratory is based on assumptions as to where a person made direct contact. In many instances a swab may be applied to an area where no contact has been made. Many swabs may therefore be submitted for DNA profiling on which no DNA is present, resulting in the loss of both time and resources by analysing such swabs. This study has developed a simple, fast, DNA-staining and fluorescence microscopy-based screening method for swabs to indicate if there is any DNA from which to generate a profile. Ten different types of swabs were tested covering the major types used (foam, cotton and nylon). Each swab was treated by: no addition of dye or DNA, addition of dye only, addition of known DNA and addition of dye and DNA. The stain used was Diamond™ Nucleic Acid Dye (DD) and fluorescence microscopy was achieved with a digital microscope equipped with a blue LED light source (480 nm) for excitation and an emission filter of 510 nm. Two types of samples were tested, either buccal swabs or swabs collected from areas touched by volunteers and all analyses were performed in triplicate. The samples were collected and retained at room temperature with time intervals of 0 day, 7 days, 14 days, 21 days, and 28 days before detection using DD staining and fluorescence microscopy. Seven of the swab types used were found to be unsuitable due to the lack of any difference in the fluorescence detected when no DNA, or only the dye, or a combination of DNA and dye were added. Three swab types (black cotton swab, Ultrafine dental applicator, and Cylinder dental applicator) were found to be much more effective for collection of DNA. Further, stained cellular material retained its fluorescence for up to 4 weeks and swabs containing cellular material that had been stored for four weeks could be stained and visualised. Additionally, DD did not affect DNA profiling. This screening method has the potential to be a routine step in a forensic laboratory to save costs of processing samples where swabs are devoid of any DNA. This technique is rapid, easy, cheap, non-destructive and safe. en_US
dc.description.sponsorship Piyamas Kanokwongnuwut was supported by the Development and Promotion of Science and Technology Talent Project (DPST), Royal Thai Government Scholarship. Funding for the work was provided by the Attorney General’s Department of South Australia via Forensic Science South Australia. en_US
dc.identifier.citation Kanokwongnuwut, P., Kirkbride, P., & Linacre, A. (2018). Visualising latent DNA on swabs. Forensic Science International, 291: 115-123. en_US
dc.identifier.doi https://doi.org/10.1016/j.forsciint.2018.08.016 en_US
dc.identifier.issn 1872-6283
dc.identifier.uri http://hdl.handle.net/2328/38397
dc.language.iso en en_US
dc.publisher Elsevier en_US
dc.rights © 2018 Elsevier. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/ en_US
dc.rights.holder © 2018 Elsevier. en_US
dc.rights.license CC-BY-NC-ND
dc.subject Diamond™ nucleic acid dye en_US
dc.subject Direct PCR en_US
dc.subject Fast screening en_US
dc.subject STR DNA profiling en_US
dc.subject Swab collection en_US
dc.title Visualising latent DNA on swabs en_US
dc.type Article en_US
local.contributor.authorOrcidLookup Linacre, Adrian Matthew Thornton: https://orcid.org/0000-0001-5890-5548 en_US
local.contributor.authorOrcidLookup Kirkbride, Kenneth Paul: https://orcid.org/0000-0001-7666-4039 en_US
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