Sustainable remediation: electrochemically assisted microbial dechlorination of tetrachloroethene-contaminated groundwater

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dc.contributor.author Patil, Sayali S
dc.contributor.author Adetutu, Eric Morakinyo (Akin)
dc.contributor.author Rochow, Jacqueline
dc.contributor.author Mitchell, James Gordon
dc.contributor.author Ball, Andrew Stephen
dc.date.accessioned 2015-09-21T04:19:23Z
dc.date.available 2015-09-21T04:19:23Z
dc.date.issued 2013-10-01
dc.description.abstract Microbial electric systems (MESs) hold significant promise for the sustainable remediation of chlorinated solvents such as tetrachlorethene (perchloroethylene, PCE). Although the bio-electrochemical potential of some specific bacterial species such as Dehalcoccoides and Geobacteraceae have been exploited, this ability in other undefined microorganisms has not been extensively assessed. Hence, the focus of this study was to investigate indigenous and potentially bio-electrochemically active microorganisms in PCE-contaminated groundwater. Lab-scale MESs were fed with acetate and carbon electrode/PCE as electron donors and acceptors, respectively, under biostimulation (BS) and BS-bioaugmentation (BS-BA) regimes. Molecular analysis of the indigenous groundwater community identified mainly Spirochaetes, Firmicutes, Bacteroidetes, and γ and δ-Proteobacteria. Environmental scanning electron photomicrographs of the anode surfaces showed extensive indigenous microbial colonization under both regimes. This colonization and BS resulted in 100% dechlorination in both treatments with complete dechlorination occurring 4 weeks earlier in BS-BA samples and up to 11.5 μA of current being generated. The indigenous non-Dehalococcoides community was found to contribute significantly to electron transfer with ∼61% of the current generated due to their activities. This study therefore shows the potential of the indigenous non-Dehalococcoides bacterial community in bio-electrochemically reducing PCE that could prove to be a cost-effective and sustainable bioremediation practice. en
dc.description.sponsorship Our sincere thanks go to the Flinders University of South Australia for providing a scholarship to the first author. en
dc.identifier.citation Patil, S.S., Adetutu, E.M., Rochow, J., Mitchell, J.G. and Ball, A.S., 2014. Sustainable remediation: electrochemically assisted microbial dechlorination of tetrachloroethene-contaminated groundwater. Microbial Biotechnology, 7(1): 54–63. doi: 10.1111/1751-7915.12089 en
dc.identifier.doi https://doi.org/10.1111/1751-7915.12089 en
dc.identifier.issn 1751-7915
dc.identifier.uri http://hdl.handle.net/2328/35552
dc.identifier.uri http://onlinelibrary.wiley.com/doi/10.1111/1751-7915.12089/abstract
dc.language.iso en en_US
dc.publisher John Wiley & Sons en_US
dc.relation http://purl.org/au-research/grants/arc/LP130100508 en_US
dc.relation.grantnumber ARC/LP130100508 en_US
dc.rights This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. en_US
dc.rights.holder © 2013 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology. en_US
dc.subject Microbiology en_US
dc.subject Groundwater en_US
dc.subject Toxins en_US
dc.title Sustainable remediation: electrochemically assisted microbial dechlorination of tetrachloroethene-contaminated groundwater en_US
dc.type Article en
local.contributor.authorOrcidLookup Mitchell, James Gordon: https://orcid.org/0000-0002-8445-0935 en_US
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