Validation of DESS as a DNA Preservation Method for the Detection of Strongyloides spp. in Canine Feces

dc.contributor.author Beknazarova, Meruyert
dc.contributor.author Millsteed, Shelby
dc.contributor.author Robertson, G
dc.contributor.author Whiley, Harriet
dc.contributor.author Ross, Kirstin Elizabeth
dc.date.accessioned 2017-07-14T00:11:21Z
dc.date.available 2017-07-14T00:11:21Z
dc.date.issued 2017-06-09
dc.description This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). en
dc.description.abstract Abstract: Strongyloides stercoralis is a gastrointestinal parasitic nematode with a life cycle that includes free-living and parasitic forms. For both clinical (diagnostic) and environmental evaluation, it is important that we can detect Strongyloides spp. in both human and non-human fecal samples. Real-time PCR is the most feasible method for detecting the parasite in both clinical and environmental samples that have been preserved. However, one of the biggest challenges with PCR detection is DNA degradation during the postage time from rural and remote areas to the laboratory. This study included a laboratory assessment and field validation of DESS (dimethyl sulfoxide, disodium EDTA, and saturated NaCl) preservation of Strongyloides spp. DNA in fecal samples. The laboratory study investigated the capacity of 1:1 and 1:3 sample to DESS ratios to preserve Strongyloides ratti in spike canine feces. It was found that both ratios of DESS significantly prevented DNA degradation compared to the untreated sample. This method was then validated by applying it to the field-collected canine feces and detecting Strongyloides DNA using PCR. A total of 37 canine feces samples were collected and preserved in the 1:3 ratio (sample: DESS) and of these, 17 were positive for Strongyloides spp. The study shows that both 1:1 and 1:3 sample to DESS ratios were able to preserve the Strongyloides spp. DNA in canine feces samples stored at room temperature for up to 56 days. This DESS preservation method presents the most applicable and feasible method for the Strongyloides DNA preservation in field-collected feces. en
dc.identifier.citation Beknazarova, M.; Millsteed, S.; Robertson, G.; Whiley, H.; Ross, K. Validation of DESS as a DNA Preservation Method for the Detection of Strongyloides spp. in Canine Feces. Int. J. Environ. Res. Public Health 2017, 14, 624. en
dc.identifier.doi https://doi.org/10.3390/ijerph14060624 en
dc.identifier.issn 1660-4601
dc.identifier.uri http://hdl.handle.net/2328/37336
dc.language.iso en
dc.publisher MDPI en
dc.rights © 2017 by the authors. en
dc.rights.holder the authors. en
dc.rights.license CC-BY
dc.subject Strongyloides stercoralis en
dc.subject Strongyloides ratti en
dc.subject Strongyloides en
dc.subject real-time PCR en
dc.subject DESS en
dc.subject DNA preservation en
dc.subject DNA degradation en
dc.subject canine feces en
dc.title Validation of DESS as a DNA Preservation Method for the Detection of Strongyloides spp. in Canine Feces en
dc.type Article en
local.contributor.authorOrcidLookup Whiley, Harriet: https://orcid.org/0000-0002-7176-9197 en_US
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