Semi-quantitative digital analysis of polymerase chain reactionelectrophoresis gel: Potential applications in low-income veterinary laboratories

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dc.contributor.author Antiabong, John F
dc.contributor.author Ngoepe, Mafora G
dc.contributor.author Abechi, Adakole S
dc.date.accessioned 2017-04-05T06:05:09Z
dc.date.available 2017-04-05T06:05:09Z
dc.date.issued 2016-09-03
dc.description The articles in Veterinary World are open access articles licensed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited. en
dc.description.abstract Aim: The interpretation of conventional polymerase chain reaction (PCR) assay results is often limited to either positive or negative (non-detectable). The more robust quantitative PCR (qPCR) method is mostly reserved for quantitation studies and not a readily accessible technology in laboratories across developing nations. The aim of this study was to evaluate a semi-quantitative method for conventional PCR amplicons using digital image analysis of electrophoretic gel. The potential applications are also discussed. Materials and Methods: This study describes standard conditions for the digital image analysis of PCR amplicons using the freely available ImageJ software and confirmed using the qPCR assay. Results and Conclusion: Comparison of ImageJ analysis of PCR-electrophoresis gel and qPCR methods showed similar trends in the Fusobacterium necrophorum DNA concentration associated with healthy and periodontal disease infected wallabies (p≤0.03). Based on these empirical data, this study adds descriptive attributes (“more” or “less”) to the interpretation of conventional PCR results. The potential applications in low-income veterinary laboratories are suggested, and guidelines for the adoption of the method are also highlighted. en
dc.identifier.citation Antiabong JF, Ngoepe MG, Abechi AS (2016) Semiquantitative digital analysis of polymerase chain reaction-electrophoresis gel: Potential applications in low-income veterinary laboratories, Veterinary World, 9(9): 935-939. en
dc.identifier.doi https://doi.org/10.14202/vetworld.2016.935-939 en
dc.identifier.issn 2231-0916
dc.identifier.uri http://hdl.handle.net/2328/37091
dc.language.iso en
dc.oaire.license.condition.license CC-BY
dc.publisher Veterinary World en
dc.rights The articles in Veterinary World are open access articles licensed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution and reproduction in any medium, provided the work is properly cited. en
dc.rights.holder The author en
dc.subject applications, en
dc.subject digital image analysis en
dc.subject ImageJ software en
dc.subject polymerase chain reaction-electrophoresis en
dc.subject polymerase chain reaction amplicon en
dc.subject quantitative polymerase chain reaction en
dc.title Semi-quantitative digital analysis of polymerase chain reactionelectrophoresis gel: Potential applications in low-income veterinary laboratories en
dc.type Article en
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