Firing patterns and functional roles of different classes of spinal afferents in rectal nerves during colonic migrating motor complexes in mouse colon

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Date
2012-08-01, 2012-08-01
Authors
Zagorodnyuk, Vladimir Petrovich
Kyloh, Melinda
Brookes, Simon Jonathan
Nicholas, Sarah J
Spencer, Nicholas John
Journal Title
Journal ISSN
Volume Title
Publisher
American Physiological Society
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Copyright © 2012 the American Physiological Society
Abstract
The functional role of the different classes of visceral afferents that innervate the large intestine is poorly understood. Recent evidence suggests that low-threshold, wide-dynamic-range rectal afferents play an important role in the detection and transmission of visceral pain induced by noxious colorectal distension in mice. However, it is not clear which classes of spinal afferents are activated during naturally occurring colonic motor patterns or during intense contractions of the gut smooth muscle. We developed an in vitro colorectum preparation to test how the major classes of rectal afferents are activated during spontaneous colonic migrating motor complex (CMMC) or pharmacologically induced contraction. During CMMCs, circular muscle contractions increased firing in low-threshold, wide-dynamic-range muscular afferents and muscular-mucosal afferents, which generated a mean firing rate of 1.53 ± 0.23 Hz (n = 8) under isotonic conditions and 2.52 ± 0.36 Hz (n = 17) under isometric conditions. These low-threshold rectal afferents were reliably activated by low levels of circumferential stretch induced by increases in length (1–2 mm) or load (1–3 g). In a small proportion of cases (5 of 34 units), some low-threshold muscular and muscular-mucosal afferents decreased their firing rate during the peak of the CMMC contractions. High-threshold afferents were never activated during spontaneous CMMC contractions or tonic contractions induced by bethanechol (100 μM). High-threshold rectal afferents were only activated by intense levels of circumferential stretch (10–20 g). These results show that, in the rectal nerves of mice, low-threshold, wide-dynamic-range muscular and muscular-mucosal afferents are excited during contraction of the circular muscle that occurs during spontaneous CMMCs. No activation of high-threshold rectal afferents was detected during CMMCs or intense contractile activity in naïve mouse colorectum.
Description
Keywords
Migrating myoelectric complex, In vitro techniques, Afferent neurons
Citation
Zagorodnyuk, V.P., Kyloh, M., Brookes, S.J.H., Nicholas, S.J. and Spencer, N.J., 2012. Firing patterns and functional roles of different classes of spinal afferents in rectal nerves during spontaneous colonic migrating motor complexes in mouse colon. American Journal of Physiology: Gastrointestinal and Liver Physiology, 303(3), G404-G411.