Microfluidic formation of core-shell alginate microparticles for protein encapsulation and controlled release

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Yu, Lei
Sun, Qi
Hui, Yue
Seth, Arjun
Petrovsky, Nikolai
Zhao, Chun-Xia
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© 2018 Elsevier Ltd.
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Elsevier Ltd.
Alginate hydrogel particles are promising delivery systems for protein encapsulation and controlled release because of their excellent biocompatibility, biodegradability, and mild gelation process. In this study, a facile microfluidic approach is developed for making uniform core-shell hydrogel microparticles. To address the challenge of protein retention within the alginate gel matrix, poly(ethyleneimine) (PEI)- and chitosan-coated alginate microparticles were fabricated demonstrating improved protein retention as well as controlled release. Furthermore, a model protein ovalbumin was loaded along with delta inulin microparticulate adjuvant into the water-core of the alginate microparticles. Compared to those microparticles with only antigen loaded, the antigen + adjuvant loaded microparticles showed a delayed and sustained release of antigen. This microfluidic approach provides a convenient method for making well-controlled alginate microgel particles with uniform size and controlled properties, and demonstrates the ability to tune the release profiles of proteins by engineering microparticle structure and properties.
© 2018 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/ This author accepted manuscript is made available following 12 month embargo from date of publication (December 2018) in accordance with the publisher’s archiving policy
Alginate microparticles, Microfluidics, Protein encapsulation, Controlled release, Chitosan
Yu, L., Sun, Q., Hui, Y., Seth, A., Petrovsky, N., & Zhao, C.-X. (2019). Microfluidic formation of core-shell alginate microparticles for protein encapsulation and controlled release. Journal of Colloid and Interface Science, 539, 497–503. https://doi.org/10.1016/ j.jcis.2018.12.075