Ablation of Sphingosine 1-Phosphate Receptor Subtype 3 Impairs Hippocampal Neuron Excitability In vitro and Spatial Working Memory In vivo

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dc.contributor.author Weth-Malsch, Daniela
dc.contributor.author Langeslag, Michiel
dc.contributor.author Beroukas, Dimitra
dc.contributor.author Zangrandi, Luca
dc.contributor.author Kastenberger, Iris
dc.contributor.author Quarta, Serena
dc.contributor.author Malsch, Philipp
dc.contributor.author Kalpachidou, Theodora
dc.contributor.author Schwarzer, Christoph
dc.contributor.author Proia, Richard L
dc.contributor.author Haberberger, Rainer Viktor
dc.contributor.author Kress, Michaela
dc.date.accessioned 2017-04-03T03:34:06Z
dc.date.available 2017-04-03T03:34:06Z
dc.date.issued 2016-11-07
dc.description This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. en
dc.description.abstract Understanding the role of the bioactive lipid mediator sphingosine 1-phosphate (S1P) within the central nervous system has recently gained more and more attention, as it has been connected to major diseases such as multiple sclerosis and Alzheimer's disease. Even though much data about the functions of the five S1P receptors has been collected for other organ systems, we still lack a complete understanding for their specific roles, in particular within the brain. Therefore, it was the aim of this study to further elucidate the role of S1P receptor subtype 3 (S1P3) in vivo and in vitro with a special focus on the hippocampus. Using an S1P3 knock-out mouse model we applied a range of behavioral tests, performed expression studies, and whole cell patch clamp recordings in acute hippocampal slices. We were able to show that S1P3 deficient mice display a significant spatial working memory deficit within the T-maze test, but not in anxiety related tests. Furthermore, S1p3 mRNA was expressed throughout the hippocampal formation. Principal neurons in area CA3 lacking S1P3 showed significantly increased interspike intervals and a significantly decreased input resistance. Upon stimulation with S1P CA3 principal neurons from both wildtype and S1P−/−3 mice displayed significantly increased evoked EPSC amplitudes and decay times, whereas rise times remained unchanged. These results suggest a specific involvement of S1P3 for the establishment of spatial working memory and neuronal excitability within the hippocampus. en
dc.identifier.citation Weth-Malsch D, Langeslag M, Beroukas D, Zangrandi L, Kastenberger I, Quarta S, Malsch P, Kalpachidou T, Schwarzer C, Proia RL, Haberberger RV and Kress M (2016) Ablation of Sphingosine 1-Phosphate Receptor Subtype 3 Impairs Hippocampal Neuron Excitability In vitro and Spatial Working Memory In vivo. Front. Cell. Neurosci. 10:258. doi: 10.3389/fncel.2016.00258 en
dc.identifier.doi https://doi.org/10.3389/fncel.2016.00258 en
dc.identifier.issn 1662-5102
dc.identifier.uri http://hdl.handle.net/2328/37053
dc.language.iso en
dc.oaire.license.condition.license CC-BY
dc.publisher Frontiers Media S.A. en
dc.rights © 2016 Weth-Malsch, Langeslag, Beroukas, Zangrandi, Kastenberger, Quarta, Malsch, Kalpachidou, Schwarzer, Proia, Haberberger and Kress. en
dc.rights.holder Weth-Malsch, Langeslag, Beroukas, Zangrandi, Kastenberger, Quarta, Malsch, Kalpachidou, Schwarzer, Proia, Haberberger and Kress. en
dc.subject sphingosine 1-phosphate en
dc.subject S1P receptor 3 en
dc.subject hippocampus en
dc.subject working memory en
dc.subject neuron excitability en
dc.title Ablation of Sphingosine 1-Phosphate Receptor Subtype 3 Impairs Hippocampal Neuron Excitability In vitro and Spatial Working Memory In vivo en
dc.type Article en
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