Identification and In Vitro Expansion of Buccal Epithelial Cells Ghaemi, Soraya Rasi Delalat, Bahman Harding, Frances Jane Irani, Yazad Williams, Keryn Anne Voelcker, Nicolas Hans 2018-09-28T01:42:09Z 2018-09-28T01:42:09Z 2018-06-01
dc.description This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. en_US
dc.description.abstract Ex vivo-expanded buccal mucosal epithelial (BME) cell transplantation has been used to reconstruct the ocular surface. Methods for enrichment and maintenance of BME progenitor cells in ex vivo cultures may improve the outcome of BME cell transplantation. However, the parameter of cell seeding density in this context has largely been neglected. This study investigates how varying cell seeding density influences BME cell proliferation and differentiation on tissue culture polystyrene (TCPS). The highest cell proliferation activity was seen when cells were seeded at 5×104 cells/cm2. Both below and above this density, the cell proliferation rate decreased sharply. Differential immunofluorescence analysis of surface markers associated with the BME progenitor cell population (p63, CK19, and ABCG2), the differentiated cell marker CK10 and connexin 50 (Cx50) revealed that the initial cell seeding density also significantly affected the progenitor cell marker expression profile. Hence, this study demonstrates that seeding density has a profound effect on the proliferation and differentiation of BME stem cells in vitro, and this is relevant to downstream cell therapy applications. en_US
dc.description.sponsorship The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: This work was supported by ‘NHMRC Australia Project grant’, grant agreement No. 595901. en_US
dc.identifier.citation Ghaemi, S.R., Delalat, B., Harding, F.J., Irani, Y., Williams, K.A. & Voelcker, N.H., (2018). Identification and In Vitro Expansion of Buccal Epithelial Cells. Cell Transplantation, 27(6): 957-966. en_US
dc.identifier.doi en_US
dc.identifier.issn 1555-3892
dc.language.iso en en_US
dc.oaire.license.condition.license CC-BY-NC-ND
dc.publisher SAGE Publications en_US
dc.relation NHMRC/595901 en_US
dc.rights © The Author(s) 2018, made available under the CC-BY-NC-ND 4.0 license: en_US
dc.rights.holder © The Author(s) 2018 en_US
dc.subject epithelial cell en_US
dc.subject cheek and mouth mucosa en_US
dc.subject cell transplant en_US
dc.subject cell proliferation en_US
dc.subject cell differentiation en_US
dc.title Identification and In Vitro Expansion of Buccal Epithelial Cells en_US
dc.type Article en
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