Identification and In Vitro Expansion of Buccal Epithelial Cells
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Date
2018-06-01
Authors
Ghaemi, Soraya Rasi
Delalat, Bahman
Harding, Frances Jane
Irani, Yazad
Williams, Keryn Anne
Voelcker, Nicolas Hans
Journal Title
Journal ISSN
Volume Title
Publisher
SAGE Publications
Rights
© The Author(s) 2018, made available under the CC-BY-NC-ND 4.0 license: http://creativecommons.org/licenses/by-nc-nd/4.0/
Rights Holder
© The Author(s) 2018
Abstract
Ex vivo-expanded buccal mucosal epithelial (BME) cell transplantation has been used to reconstruct the ocular surface. Methods for enrichment and maintenance of BME progenitor cells in ex vivo cultures may improve the outcome of BME cell transplantation. However, the parameter of cell seeding density in this context has largely been neglected. This study investigates how varying cell seeding density influences BME cell proliferation and differentiation on tissue culture polystyrene (TCPS). The highest cell proliferation activity was seen when cells were seeded at 5×104 cells/cm2. Both below and above this density, the cell proliferation rate decreased sharply. Differential immunofluorescence analysis of surface markers associated with the BME progenitor cell population (p63, CK19, and ABCG2), the differentiated cell marker CK10 and connexin 50 (Cx50) revealed that the initial cell seeding density also significantly affected the progenitor cell marker expression profile. Hence, this study demonstrates that seeding density has a profound effect on the proliferation and differentiation of BME stem cells in vitro, and this is relevant to downstream cell therapy applications.
Description
This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Keywords
epithelial cell, cheek and mouth mucosa, cell transplant, cell proliferation, cell differentiation
Citation
Ghaemi, S.R., Delalat, B., Harding, F.J., Irani, Y., Williams, K.A. & Voelcker, N.H., (2018). Identification and In Vitro Expansion of Buccal Epithelial Cells. Cell Transplantation, 27(6): 957-966.