National Health and Medical Research Council (NHMRC)

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This is a collection of NHMRC-funded research publications authored by Flinders academics.

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Now showing 1 - 6 of 358
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    Finding My Way: results of a multicentre RCT evaluating a web-based self-guided psychosocial intervention for newly diagnosed cancer survivors
    (Springer Verlag., 2018-11-09) Beatty, Lisa Jane ; Kemp, Emma ; Coll, Joseph R ; Turner, Jane ; Butow, Phyllis N ; Milne, Donna ; Yates, Patsy ; Lambert, Sylvie D ; Wootten, Addie ; Yip, Desmond ; Koczwara, Bogda
    Purpose This multicentre randomised controlled trial examined the efficacy of Finding My Way (FMW), a 6-week/6-module online self-guided psychotherapeutic intervention for newly diagnosed curatively treated cancer survivors, in reducing cancer-related distress and improving quality of life compared to an online attention control. Methods Participants were randomised on a 1:1 ratio using a gender-stratified block design to intervention (n = 94) or attention control (n = 97), and were blinded to condition. Assessments were completed at baseline (T0), post-intervention (T1), 3 months (T2), and 6 months (T3) post-intervention. Mixed model repeated measures analyses examined differences between groups for cancer-specific distress (primary outcome) and general distress, quality of life (QoL), coping, and health service utilisation (secondary outcomes). Results While both groups reported reduced cancer-specific and general distress over time, between-group differences were not significant. Intervention participants reported lower total health service utilisation and supportive care utilisation post-intervention than controls (total HS use: between-group mean difference = − 1.07 (− 1.85 to − 0.28); supportive care use: between-group mean difference = − 0.64 (− 1.21 to − 0.06)) and significantly higher emotional functioning at 3 months (between-group mean difference = 7.04 (0.15 to 13.9)). At 6 months, the supportive care utilisation finding reversed (between-group mean difference = 0.78 points (0.19 to 1.37). Across remaining QoL and coping outcomes, no significant group differences emerged. Conclusions While both groups experienced reductions in distress, between-group differences were not significant. This contrasts with the significantly improved emotional functioning observed in FMW participants at 3 months and the short-term reductions in health service utilisation. Long-term increases in supportive care service utilisation suggest FMW only met needs while being actively used.
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    Analysis combining correlated glaucoma traits identifies five new risk loci for open-angle glaucoma
    (Nature Publishing Group, 2018-02-18) Gharahkhani, Puya ; Burdon, Kathryn Penelope ; Cooke-Bailey, Jessica N ; Hewitt, Alex W ; Law, Matthew H ; Pasquale, Louis R ; Kang, Jae Hee ; Haines, Jonathan L ; Souzeau, Emmanuelle ; Zhou, Tiger ; Siggs, Owen M ; Landers, John ; Awadalla, Mona S ; Sharma, Shiwani ; Mills, Richard Arthur ; Ridge, Bronwyn ; Lynn, David J ; Casson, Robert J ; Graham, Stuart L ; Goldberg, Ivan ; White, Andrew J ; Healey, Paul R ; Grigg, John RB ; Lawlor, Mitchell ; Mitchell, Paul ; Ruddle, Jonathan B ; Coote, Michael A ; Walland, Mark ; Best, Stephen ; Vincent, Andrea ; Gale, Jesse ; Radford-Smith, Graham ; Whiteman, David C ; Montgomery, Grant W ; Martin, Nicholas G ; Mackey, David A ; Wiggs, Janey L ; MacGregor, Stuart ; Craig, Jamie E ; Neighborhood Consortium
    Open-angle glaucoma (OAG) is a major cause of blindness worldwide. To identify new risk loci for OAG, we performed a genome-wide association study in 3,071 OAG cases and 6,750 unscreened controls, and meta-analysed the results with GWAS data for intraocular pressure (IOP) and optic disc parameters (the overall meta-analysis sample size varying between 32,000 to 48,000 participants), which are glaucoma-related traits. We identified and independently validated four novel genome-wide significant associations within or near MYOF and CYP26A1, LINC02052 and CRYGS, LMX1B, and LMO7 using single variant tests, one additional locus (C9) using gene-based tests, and two genetic pathways - “response to fluid shear stress” and “abnormal retina morphology” - in pathway-based tests. Interestingly, some of the new risk loci contribute to risk of other genetically-correlated eye diseases including myopia and age-related macular degeneration. To our knowledge, this study is the first integrative study to combine genetic data from OAG and its correlated traits to identify new risk variants and genetic pathways, highlighting the future potential of combining genetic data from genetically-correlated eye traits for the purpose of gene discovery and mapping.
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    DNA methylation at the 9p21 glaucoma susceptibility locus is associated with normal-tension glaucoma
    (Taylor & Francis Group, 2017-12-21) Burdon, Kathryn Penelope ; Awadalla, Mona S ; Mitchell, Paul ; Wang, Jie Jin ; White, Andrew J ; Keane, Miriam Claire ; Souzeau, Emmanuelle ; Graham, Stuart L ; Goldberg, Ivan ; Healey, Paul R ; Landers, John ; Mills, Richard Arthur ; Best, Stephen ; Hewitt, Alex W ; Sharma, Shiwani ; Craig, Jamie E
    Purpose: Recent genome-wide association studies reported strong association of genetic variation at the CDKN2B/CDKN2B-AS1 locus on 9p21 with normal-tension glaucoma (NTG) in multiple populations. The mechanism by which this locus causes disease remains to be elucidated. We investigated the association of DNA methylation of CpG islands at this locus with NTG. Methods: We conducted a retrospective case–control study of 178 NTG cases and 202 unaffected controls from Australia. CDKN2B and CDKN2B-AS1 promoter methylation was measured quantitatively using the MassCleave assay, and assessed for association with the disease, and the genotype of the associated risk variants using IBM SPSS statistics 22.0 CpG sites at which methylation status was associated with NTG were validated using pyrosequencing. Results: We identified one CpG site (F1:13–14) in the CDKN2B promoter which showed significant association with NTG (p = 0.001). The association was highly significant in female cases (p = 0.006) but not in male cases (p = 0.054). The association was validated using an independent method confirming the likely association of DNA methylation with NTG in females (p = 0.015), but not in males (p = 0.497). In addition, methylation at CpG sites in CDKN2B was also associated with genotype at rs1063192, which is known to confer risk for NTG. Conclusion: This study reveals an association of methylation status in the CDKN2B promoter with NTG, particularly in females. This suggests that the observed genetic association with the disease at this locus could be in part due to epigenetic mechanisms, and is likely to be independent of the association of nonsynonymous coding variation within the gene.
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    Rare, Potentially Pathogenic Variants in ZNF469 Are Not Enriched in Keratoconus in a Large Australian Cohort of European Descent
    (Investigative Ophthalmology and Visual Science, 2017-12) Lucas, Sionne E ; Zhou, Tiger ; Blackburn, Nicholas B ; Mills, Richard Arthur ; Ellis, Jonathan ; Leo, Paul ; Souzeau, Emmanuelle ; Ridge, Bronwyn ; Charlesworth, Jac C ; Brown, Matthew A ; Lindsay, Richard ; Craig, Jamie E ; Burdon, Kathryn Penelope
    Purpose: The Zinc Finger Protein 469 (ZNF469) gene has been proposed as a candidate gene for keratoconus due to the association of an upstream polymorphism (rs9938149) with the disease in two independent studies, and the role of the gene in the autosomal recessive disease Brittle Cornea Syndrome. Coding variants in ZNF469 have been assessed for association with keratoconus in several small studies, with conflicting results. We assessed rare, potentially pathogenic variants in ZNF469 for enrichment in keratoconus patients in a cohort larger than all previous studies combined. Methods: ZNF469 was sequenced in 385 Australian keratoconus patients of European descent, 346 population controls, and 230 ethnically matched screened controls by either whole exome sequencing or targeted gene sequencing. The frequency of rare and very rare potentially pathogenic variants was compared between cases and controls using χ2 or Fisher's exact tests and further explored using a gene based test (Sequence Kernel Association Test [SKAT]), weighting on the rarity of variants. Results: A total of 49 rare, including 33 very rare, potentially pathogenic variants were identified across all groups. No enrichment of rare or very rare potentially pathogenic variants in ZNF469 was observed in our cases compared to the control groups following analysis using χ2 or Fisher's exact tests. This finding was further supported by the SKAT results, which found no significant difference in the frequency of variants predicted to be damaging between cases and either control group (P = 0.06).
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    Contribution of Mutations in Known Mendelian Glaucoma Genes to Advanced Early-Onset Primary Open-Angle Glaucoma
    (Association for Research in Vision and Ophthalmology, Inc., 2017-03) Zhou, Tiger ; Souzeau, Emmanuelle ; Siggs, Owen M ; Landers, John ; Mills, Richard Arthur ; Goldberg, Ivan ; Healey, Paul R ; Graham, Stuart L ; Hewitt, Alex W ; Mackey, David A ; Galanopoulos, Anna ; Casson, Robert J ; Ruddle, Jonathan B ; Ellis, Jonathan ; Leo, Jonathan ; Brown, Matthew A ; MacGregor, Stuart ; Sharma, Shiwani ; Burdon, Kathryn Penelope ; Simmonds, Jamie E
    Purpose: Primary open-angle glaucoma (POAG) and primary congenital glaucoma (PCG) with Mendelian inheritance are caused by mutations in at least nine genes. Utilizing whole-exome sequencing, we examined the disease burden accounted for by these known Mendelian glaucoma genes in a cohort of individuals with advanced early-onset POAG. Methods: The cases exhibited advanced POAG with young age of diagnosis. Cases and examined local controls were subjected to whole-exome sequencing. Nine hundred ninety-three previously sequenced exomes of Australian controls were called jointly with our dataset. Qualifying variants were selected based on predicted pathogenicity and rarity in public domain gene variant databases. Case–control mutational burdens were calculated for glaucoma-linked genes. Results: Two hundred eighteen unrelated POAG participants and 103 nonglaucomatous controls were included in addition to 993 unexamined controls. Fifty-eight participants (26.6%) harbored rare potentially pathogenic variants in known glaucoma genes. Enrichment of qualifying variants toward glaucoma was present in all genes except WDR36, in which controls harbored more variants, and TBK1, in which no qualifying variants were detected in cases or controls. After multiple testing correction, only MYOC showed statistically significant enrichment of qualifying variants (odds ratio [OR] = 16.62, P = 6.31×10−16). Conclusions: Rare, potentially disease-causing variants in Mendelian POAG genes that showed enrichment in our dataset were found in 22.9% of advanced early-onset POAG cases. MYOC variants represented the largest monogenic cause in POAG. The association between WDR36 and POAG was not supported, and the majority of POAG cases did not harbor a potentially disease-causing variant in the remaining Mendelian genes.
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    Partial duplication of the CRYBB1-CRYBA4 locus is associated with autosomal dominant congenital cataract
    (Nature Publishing Group, 2017-03-08) Siggs, Owen M ; Javadiyan, Shahrbanou ; Sharma, Shiwani ; Souzeau, Emmanuelle ; Lower, Karen Marie ; Taranath, Deepa A ; Black, J A ; Pater, John Brian ; Willoughby, John G ; Burdon, Kathryn Penelope ; Craig, Jamie E
    Congenital cataract is a rare but severe paediatric visual impediment, often caused by variants in one of several crystallin genes that produce the bulk of structural proteins in the lens. Here we describe a pedigree with autosomal dominant isolated congenital cataract and linkage to the crystallin gene cluster on chromosome 22. No rare single nucleotide variants or short indels were identified by exome sequencing, yet copy number variant analysis revealed a duplication spanning both CRYBB1 and CRYBA4. While the CRYBA4 duplication was complete, the CRYBB1 duplication was not, with the duplicated CRYBB1 product predicted to create a gain of function allele. This association suggests a new genetic mechanism for the development of isolated congenital cataract.