Browsing Australian Research Council (ARC) by Subject "Analytical chemistry"
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ItemNon-toxic luminescent carbon dot/poly(dimethylacrylamide) nanocomposite reagent for latent fingermark detection synthesized via surface initiated reversible addition fragmentation chain transfer polymerization(John Wiley & Sons, 2015-01-13) Dilag, Jessirie; Kobus, Hilton John; Yu, Yang; Gibson, Christopher; Ellis, Amanda VeraHere, non-toxic luminescent carbon nanoparticles, namely carbon dots (C-dots), were facilely synthesized via a one-pot hydrothermal route. Raman, Fourier transform infrared, fluorescence, carbon NMR and X-ray photoelectron spectroscopies revealed that the C-dots possessed a graphitic-like core with an oxidized surface. The oxidized surface of the C-dots allowed for functionalization of the C-dots with a 2-methyl-2-[(dodecylsulfanylthiocarbonyl)sulfanyl]propanoic acid chain transfer agent. Poly(N,N-dimethylacrylamide) (p(DMA)) was then grafted from the C-dot surface via surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization. The resulting luminescent C-dot/polymer nanocomposite, C-dot/p(DMA), was analyzed using UV−visible and fluorescence spectrometry verifying that the functionalized surface was responsible for the C-dots' luminescence. This C-dot/p(DMA) nanocomposite was water soluble and was used as a solution for the luminescent detection of latent fingermarks deposited on non-porous aluminium foil substrates. ItemRecent developments in nucleic acid identification using solid-phase enzymatic assays(Springer Verlag, 2014-02-04) Khodakov, Dmitriy A; Ellis, Amanda VeraThis review (containing 101 references) covers recent achievements in the development of new approaches for enzymatically assisted detection of nucleic acids on microarrays. We discuss molecular techniques including the polymerase chain reaction, reverse transcription, allele specific primer extension and a range of isothermal techniques for the amplification and discrimination of nucleic acids. This also includes their implementation into microfluidic systems. These techniques all show great promise for use in the life sciences by allowing for high throughput, cost effective and highly sensitive and specific analysis of nucleic acids. Importantly, they can be potentially integrated into personalized and point-of-care medicine. ItemSequence selective capture, release and analysis of DNA using a magnetic microbead-assisted toehold-mediated DNA strand displacement reaction(Royal Society of Chemistry, 2014-05-15) Khodakov, Dmitriy A; Khodakova, Anastasia S; Linacre, Adrian Matthew Thornton; Ellis, Amanda VeraThis paper reports on the modification of magnetic beads with oligonucleotide capture probes with a specially designed pendant toehold (overhang) aimed specifically to capture double-stranded PCR products. After capture, the PCR products were selectively released from the magnetic beads by means of a toehold-mediated strand displacement reaction using short artificial oligonucleotide triggers and analysed using capillary electrophoresis. The approach was successfully shown on two genes widely used in human DNA genotyping, namely human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO) and amelogenin.